Journal: Med (New York, N.y.)

Article Title: Early T cell and binding antibody responses are associated with COVID-19 RNA vaccine efficacy onset

doi: 10.1016/j.medj.2021.04.003

Figure Lengend Snippet:

Article Snippet: No-GFP-CD16.NK-92 cell line (High affinity 176V) , ATCC , PTA-6967.

Techniques: Virus, Recombinant, Staining, Luminex, Neutralization, Generated, Software

Journal: Scientific Reports

Article Title: Association of Cyclin Dependent Kinase 10 and Transcription Factor 2 during Human Corneal Epithelial Wound Healing in vitro model

doi: 10.1038/s41598-019-48092-6

Figure Lengend Snippet: ( A ) Human corneal epithelial cells (HCEC) were subjected to in vitro scratch assay. Representative images from scratch wound healing assay of HCEC showing time course of corneal re-epithelialization (abrasion 1 mm) in vitro model at different time intervals (0, 6, 12, 18 & 24 hours) after post wounding. Scratch wounds were made in confluent cultures of corneal epithelial cells. The red lines define the area lacking cells where as arrows indicating the movement of cells towards closing the wound. The images were analyzed by Image J software (National Institutes of Health [NIH], Bethesda, MD, USA) with Scale bar = 100 µm. Images were captured at 4X magnification using camera-equipped inverted microscope (Olympus Onvented, DSR-012). ( B ) In vitro wound healing of migrating corneal epithelia in confluent monolayer of HCEC showing linear phase of wound healing at time intervals 6, 12, 18 and 24 hours while wound was closed at 24 hours of post wounding. Cellular migration was calculated using one way ANOVA by GraphPad (7.0) with significance of p < 0.001. Each value is representing three individual experiments, error bars indicates SDM.

Article Snippet: The Human corneal epithelial cell (HCEC), were purchased from American Type Culture Collection (ATCC, Rockville, MD, USA).

Techniques: In Vitro, Wound Healing Assay, Software, Inverted Microscopy, Migration

Journal: Scientific Reports

Article Title: Association of Cyclin Dependent Kinase 10 and Transcription Factor 2 during Human Corneal Epithelial Wound Healing in vitro model

doi: 10.1038/s41598-019-48092-6

Figure Lengend Snippet: Western Blot analysis detected expression of cdk10 and ETS2 in HCEC: ( A) The expression level of cdk10 and ETS2 at active hours of migration (18 hours) related to non-migrating sample with beta- Actin as loading control. lane 1 NM, non-migrating; lane 2, migrating at 18 hrs. (B) Quantification and intensity measurement of relative protein expression were analyzed by Quantity One software (Bio-Rad, USA). Histograms are generated using GraphPad Prism software (7.04).Values are expressed as ± SD, significance (P < 0.005) was calculated using one way ANOVA test statistically.

Article Snippet: The Human corneal epithelial cell (HCEC), were purchased from American Type Culture Collection (ATCC, Rockville, MD, USA).

Techniques: Western Blot, Expressing, Migration, Control, Software, Generated

Journal: Human Gene Therapy

Article Title: LyP-1-Modified Oncolytic Adenoviruses Targeting Transforming Growth Factor β Inhibit Tumor Growth and Metastases and Augment Immune Checkpoint Inhibitor Therapy in Breast Cancer Mouse Models

doi: 10.1089/hum.2020.078

Figure Lengend Snippet: LyP-1 receptor (p32) and/or TGFβ-1 expression in breast cancer cell lines and patients. (A) LyP-1 receptor (p32) expression in human and mouse breast cancer cells by immunofluorescence staining. In MDA-MB-231 and 4T1 cells, LyP-1 receptors were localized more on plasma membrane (white arrows) and endoplasmic reticulum-like membrane networks (white triangular arrowheads). In MCF-7 cells, they were more enriched in Golgi-like juxtanuclear compartments (white arrows). Scale bar = 25 μm. (B) LyP-1 receptor expression in breast cancer cell lines by Western blot. Note that mouse LyP-1 receptors in 4T1 cells have a slightly different banding pattern from those in human breast cancer cells. All experiments were repeated three times. (C) Representative images of LyP-1 receptor and TGFβ-1 expression in breast cancer patient samples. Yellow arrows point to positive p32 IHC staining in tumor cells; red open arrows point to TGFβ-1 staining in tumor stroma. Magnification is indicated by scale bars. IHC, immunohistochemistry; TGFβ-1, transforming growth factor β-1.

Article Snippet: Human mammary tumor cell lines, MCF-7 (ATCC, Manassas, VA), MDA-MB-231 (ATCC), and MDA-MB-231-luc2, 16 and the mouse mammary tumor cell line, 4T1 (ATCC), were maintained as described earlier.

Techniques: Expressing, Immunofluorescence, Staining, Clinical Proteomics, Membrane, Western Blot, Immunohistochemistry

Journal: Journal of Hematology & Oncology

Article Title: Challenges and advances in clinical applications of mesenchymal stromal cells

doi: 10.1186/s13045-021-01037-x

Figure Lengend Snippet: The tests of modified MSCs using CRISPR-Cas9 technology

Article Snippet: Immortalized human bone marrow MSC cell line (ATCC PCS-500–041) , PUMILIO2 (PUM2) , Depletion of PUM2 blocks MSC adipogenesis and enhances osteogenesis. PUM2 works as a negative regulator on the 3′ UTRs of JAK2 and RUNX2 via direct binding. CRISPR/CAS9-mediated gene silencing of Pum2 inhibited lipid accumulation and excessive bone formation , [ ] .

Techniques: Modification, CRISPR, Functional Assay, Synthesized, In Vitro, Transfection, Expressing, Transplantation Assay, Inhibition, Binding Assay